AAMC FL Practice Exam 2026 – 400 Free Practice Questions to Pass the Exam

Question: 1 / 400

In a Linweaver-Burk plot, what does the y-intercept represent?

1/Km of the enzyme

Vmax of the enzyme

In a Lineweaver-Burk plot, the y-intercept is actually indicative of the maximum velocity (Vmax) of the enzyme. This graph is a double-reciprocal plot used to determine kinetic parameters of enzymes, where the x-axis represents 1/[S] (the inverse of the substrate concentration) and the y-axis represents 1/V0 (the inverse of the initial velocity).

When plotting the data, Vmax appears in the equation of the line as part of the relationship established by the Michaelis-Menten kinetics, allowing for clear determination of the maximum rate of the reaction. Thus, the correct interpretation of the y-intercept directly correlates to the maximum reaction velocity of the enzyme.

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The slope of the line

The half-life of the substrate

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